Device
Part:BBa_K2230008:Design
Designed by: Yi-Lun Huang Group: iGEM17_Mingdao (2017-10-19)
Pcar-RBS-aeBlue/pSB1A3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
No
Source
Pcar-RBS-aeBlue was amplified from CP29-RBS-aeBlue (BBa_K1033280) using a primer with Pcar-RBS (B0034) sequence. The PCR product was ligated to pSB1A3 cut by XbaI and PstI.